FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16837
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA402023.875987408683258No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA357621.238938053097346No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG290817.27148541901764No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA10616.301597671794262No Hit
GCAAAAGTTCTCAGGGATGTCCACCTTGGGCTGTTGTGAGGATTAAATGA2851.6927005998693356No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2541.5085822890063552No Hit
GCAAAAGTTCTCAGGGATGTCCTGACTGGTTTTGTTGCTAGAAACCTTTA1711.0156203599216012No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1310.7780483459048524No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1250.7424125438023401No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT1120.6652016392468967No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT1090.6473837381956405No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCTTG720.4276296252301479No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA720.4276296252301479No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG670.3979331234780543No Hit
GCAAAAGTTCTCAGGGATGTCCGAGCCCTCAGGGTCCTCCCCGGTGGCTG630.3741759220763794No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA630.3741759220763794No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA610.36229732137554194No Hit
GCAAAAGTTCTCAGGGATGTCCATGACTAGTCACCCCTGCTTTCCAGTGA600.35635802102512326No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG570.33854011997386707No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA550.3266615192730296No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG510.30290431787135474No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG500.296965017520936No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA450.2672685157688424No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.25538991506800496No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA430.25538991506800496No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA420.24945061471758628No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT410.24351131436716753No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT380.2256934133159114No Hit
GCAAAAGTTCTCAGGGATGTCTGAGCCCTCAGGGTCCTCCCCGGTGGCTG380.2256934133159114No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG380.2256934133159114No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA360.21381481261507396No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT320.19005761121339906No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT320.19005761121339906No Hit
GCAAAAGTTCTCAGGGATGTCCCAGCGCTGCTCCCACTGCACCAGGCTCG310.18411831086298033No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA310.18411831086298033No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG280.16630040981172417No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA280.16630040981172417No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTTCCAGTG250.148482508760468Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGCTGCAT240.1425432084100493No Hit
GCAAAAGTTCTCAGGGATGTCCCGGCCAATGGAATTTCGAGAAAGCTTTG240.1425432084100493No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT240.1425432084100493Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.13660390805963057No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT220.13066460770921184No Hit
GCAAAAGTTCTCAGGGATGTGCAAGCAGGGAGATGAGGTTGTAAAGTTAA220.13066460770921184No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT210.12472530735879314No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT210.12472530735879314No Hit
GTGCTGTCTCTCTTTGATGGACACTGGCAGTGCCTCCTTCTCTTTTGGTT200.11878600700837441No Hit
GTGCTGTCTCTCTTTGATGCAACCCTGTGATGATTGATGCCTGCATAGAC190.1128467066579557No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA180.10690740630753698No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG180.10690740630753698No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC180.10690740630753698No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA180.10690740630753698No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.10096810595711825No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG170.10096810595711825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)