FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18232
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA556830.539710399297938No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA518728.449978060552873No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT370720.332382623957876No Hit
GTGCTGTCTCTCTTTGATGGAGGCGGCGGTAATGGCGGATGGTGGGTTGT1770.9708205353225099No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1090.5978499341816587No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1050.5759104870557262No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA980.5375164545853445No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA790.4333040807371655No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCACTGCTTCC760.4168494953927161Clontech Universal Primer Mix Long (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG720.39491004826678366No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCACCCCATGT710.38942518648530056No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA590.32360684510750326No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC570.3126371215445371No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG540.2961825362000878No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA480.26327336551118913No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA430.2358490566037736No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC410.22487933304080734No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT400.2193944712593243No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC390.21390960947784118No Hit
GTGCTGTCTCTCTTTGATGGACGGGGAGTAGGGGAAGAAAGATCTGTTGG340.18648530057042562No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA320.17551557700745943No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG280.153576129881527No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT270.1480912681000439No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGATCCAGGAGTGGGGCCCATT260.14260640631856078No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACTGCTTCC260.14260640631856078Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC250.13712154453707764No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGATCCAGGAGTGGGGCCCATT240.13163668275559456No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.12615182097411146No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.12615182097411146No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA230.12615182097411146No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.12066695919262835No Hit
GTGCTGTCTCTCTTTGATGGAATCGGCGGTAATGGCGGATGGTGGGTTGT210.11518209741114523No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA200.10969723562966215No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCCCATGTACT190.10421237384817902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)