FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523
Total Bases220.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA25816.940249507550888No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG17411.424819435325016No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT1469.58634274458306No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1399.12672357189757No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG352.298095863427446No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG342.2324359816152333No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG332.1667760998030205No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT332.1667760998030205No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT291.9041365725541695No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA281.8384766907419567No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA281.8384766907419567No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC261.7071569271175313No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG261.7071569271175313No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA191.247537754432042No Hit
GTGCTGTCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAGT181.1818778726198294No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT171.1162179908076166No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA140.9192383453709784No Hit
GTGCTGTCTCTCTTTGATGCTGCATAGACCAAGTCTCTGCTACCGTAGTA120.7879185817465528No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGCTGCATAGACC120.7879185817465528No Hit
GTGCTGTCTCTCTTTGATGGAACACTGACGACATGGTTCTACAGGATTCG110.7222586999343401No Hit
GTGCTGTCTCTCTTTGATGGAATCCTGACGACATGGTTCTACAGGATTCG110.7222586999343401No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA100.6565988181221274No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA90.5909389363099147No Hit
GTGCCTCCTTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG90.5909389363099147No Hit
GTGCTGTCTCTCTTTGATTGCTGCATAGACCAAGTCTCTGCTACCGTAGT80.5252790544977018No Hit
GTGCTGTCTCTCTTTGATGGCACTGACGACATGGTTCTACAGGATTCGTG80.5252790544977018No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCCTGCATAGACCA80.5252790544977018No Hit
GTGCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG80.5252790544977018No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT70.4596191726854892Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG70.4596191726854892No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG60.3939592908732764No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGTTGCTGCATA60.3939592908732764No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG60.3939592908732764No Hit
GTGCCTCCTTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG60.3939592908732764No Hit
GCAAAAGTTCTCAGGGATGTGATGATTGATGCCTGCATAGACCAAGTCTC40.2626395272488509No Hit
GTGCTGTCTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAGT40.2626395272488509No Hit
GTGCTGTCTCTCTTTGATGGAATCACTGACGACATGGTTCTACAGGATTC40.2626395272488509No Hit
GTGCTGTCTCTCTTTGATGGAATCCACTGACGACATGGTTCTACAGGATT40.2626395272488509No Hit
GTGCTGTCTCTCTTTGATGCCTGCATAGACCAAGTCTCTGCTACCGTAGT40.2626395272488509No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTGGTTGCTGCATA40.2626395272488509No Hit
GTGCTGTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAGTA40.2626395272488509No Hit
GTGCTGTCTCTCTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAGTA40.2626395272488509No Hit
GCAAAAGTTCTCAGGGATGGCCTCCTTCTCTTTTGGTTGCTGCATAGACC30.1969796454366382No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGCTGCAT30.1969796454366382No Hit
GTGCCTCCTTCTCTTTTGGTTCTGCATAGACCAAGTCTCTGCTACCGTAG30.1969796454366382No Hit
GCAAAAGTTCTCAGGGATGTCCCCTTCTCTTTTGGTTGCTGCATAGACCA30.1969796454366382No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACAAAGTCTCTG30.1969796454366382No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG30.1969796454366382No Hit
GTGCTGTCTCTCTTTGATGATTGATGCCTGCATAGACCAAGTCTCTGCTA30.1969796454366382No Hit
GCAAAAGTTCTCGGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGCCCCCATGT20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTCTCTTTTGGTTGCTGCATA20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTGCTGCATAGAC20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC20.13131976362442546Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGATCTGCTGCTTGACAGCGGGATCCAGGGCC20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGAATCGACATGGTTCTACAGGATTCGTGCCT20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGTTGATGCCTGCATAGACCAAGTCTCTGCTA20.13131976362442546No Hit
GCAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCATA20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCCTGATGGGTGGTAGTCGATGGTCATCCCC20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGAATTACACTGACGACATGGTTCTACAGGAT20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCGATTACGAACCAGCCTATTCCAACCCTGT20.13131976362442546No Hit
GCAAAAGTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCATA20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCCCCAGTTGTATGATTGATGCCTGCATAGA20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCCCAGTTGTATGATTGATGCCTGCATAGAC20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCGATGCCTCCTTCTCTTTTGGTTGCTGCAT20.13131976362442546No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGACTGCATAGACCAAG20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGAATCTGGTTCTACAGGATTCGTGCCTCCTT20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA20.13131976362442546Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCATA20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCCT20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGAATCGTGCCTCCTTCTCTTTTGGTTGCTGC20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCTCCTTCTCTTTTGGTTGCTGCATAGACCA20.13131976362442546No Hit
GTGCCCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG20.13131976362442546No Hit
GTGCCTCCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAGT20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCGA20.13131976362442546No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC20.13131976362442546No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA20.13131976362442546Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)