FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17256
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT788345.68266110338433No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA301317.46059341678257No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA16219.393834028743624No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT13457.794390356977283No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT1500.8692628650904033No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC720.4172461752433936No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT630.3650904033379694No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC560.3245248029670839No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.2955493741307371No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG390.22600834492350488No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT390.22600834492350488No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC380.2202132591562355No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG380.2202132591562355No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA350.20282800185442745No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG320.18544274455261936No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT310.17964765878535002No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA310.17964765878535002No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT290.1680574872508113No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA290.1680574872508113No Hit
GCATCAATCATCACAGGGTTGGAGGAAAGCTAGAATGAGTTGTGTCCTGG280.16226240148354196No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA280.16226240148354196No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA270.1564673157162726No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG260.15067222994900326No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA240.13908205841446453No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.13908205841446453No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.1332869726471952No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT220.12749188687992583No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT200.11590171534538712No Hit
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA200.11590171534538712No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT200.11590171534538712No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC200.11590171534538712No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC180.1043115438108484No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT180.1043115438108484No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG180.1043115438108484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)