FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15002
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA361424.090121317157713No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG309220.610585255299295No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA237215.811225169977336No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTCGCGCCTG184512.298360218637514No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA11547.6923076923076925No Hit
CTCCAAGTAACGGTGCTGTCTGTTGGCTGGTGCAGGGCTCCTGGTGCTGA2071.3798160245300628No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1400.9332089054792695No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT930.6199173443540861No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG630.4199440074656713No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG630.4199440074656713No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTCGCGCCTGT540.35995200639914676No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT490.3266231169177443No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.2866284495400613No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG410.2732968937475003No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.26663111585121985No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.25329956005865883No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG340.22663644847353684No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA330.21997067057725636No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA330.21997067057725636No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT290.19330755899213437No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA290.19330755899213437No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT240.15997866951073192No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT230.1533128916144514No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.1466471137181709No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT210.13998133582189043No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTCGCGCCTGT210.13998133582189043No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.13331555792560992No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTCGCGCCTGT200.13331555792560992No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG200.13331555792560992No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA190.12664978002932942No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT170.11331822423676842No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA160.10665244634048793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)