FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31194
Total Bases4.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA1166937.40783484003334No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA848327.19433224338014No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA605319.404372635763288No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1930.6187087260370584No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1710.5481823427582228No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1310.41995255497852146No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG1200.3846893633391037No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1180.3782778739501186No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCCCCTGGTGCTGA1100.35263191639417835No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG1010.3237802141437456No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC780.2500480861704174No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA720.2308136180034622No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG700.22440212861447717No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC670.21478489453099955No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG630.2019619157530294No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA600.19234468166955185No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA540.17311021350259664No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA530.16990446880810411No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA450.14425851125216388No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.13784702186317882No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA430.13784702186317882No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG410.13143553247419373No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGACTCCTGGTGCTGA410.13143553247419373No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA400.12822978777970123No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA390.1250240430852087No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC380.12181829839071616No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA340.10899531961274604No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA340.10899531961274604No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAGTACCGTCTCCTCCTCAAATCGCT330.10578957491825351No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA330.10578957491825351No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG320.10258383022376098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)