FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28658
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1637957.153325423965384No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA676923.619931607230093No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAAGCCCTGGGCTTCACTTACTCT2480.8653779049480075No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1680.5862237420615535No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA1540.537371763556424No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1500.5234140554121013No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT1260.4396678065461651No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT1240.43268895247400374No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1200.4187312443296811No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1180.41175239025751975No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1110.38732640100495497No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1080.376858119896713No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1050.3663898387884709No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA710.24774931956172797No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT680.23728103845348592No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC600.20936562216484056No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT580.2023867680926792No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT530.1849396329122758No Hit
CAACCAAAAGAGAAGGAGGCACTTTCTCCCCGCAGATCGGCCTGGCGCAG520.18145020587619512No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC490.1709819247679531No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA450.1570242166236304No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT430.15004536255146905No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT430.15004536255146905No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA420.14655593551538837No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA400.13957708144322703No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT400.13957708144322703No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT400.13957708144322703No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT380.13259822737106566No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT380.13259822737106566No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT380.13259822737106566No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT380.13259822737106566No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC360.12561937329890432No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.12561937329890432No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT350.12212994626282364No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA340.11864051922674296No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT320.11166166515458162No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA310.10817223811850094No Hit
CAACCAAAAGAGAAGGAGGCACTGAGCGACCCCGTGGGCACCTGCTACCT300.10468281108242028No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT290.1011933840463396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)