FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18187
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT751341.30972672788255No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT396621.80678506625612No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA230212.657392643096719No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA3662.012426458459339No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT2291.2591411447737395No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1300.7147962830593281No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1200.6598119535932259No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1090.5993291911805135No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA1090.5993291911805135No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT560.3079122450101721No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA530.2914169461703415No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT500.2749216473305108No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC480.26392478143729037No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA440.2419310496508495No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG410.22543575081101888No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT400.21993731786440868No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA390.21443888491779842No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT380.2089404519711882No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG360.19794358607796778No Hit
CAACCAAAAGAGAAGGAGGCACACTGGAGAGAAACCATACAAATGTGAAG330.1814482872381371No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG270.14845768955847582No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA270.14845768955847582No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.14295925661186562No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT250.1374608236652554No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC250.1374608236652554No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG240.13196239071864518No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG240.13196239071864518No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.12646395777203498No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC200.10996865893220434No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT200.10996865893220434No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.1044702259855941No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.1044702259855941No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.1044702259855941No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT190.1044702259855941No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.1044702259855941No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)