FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46353
Total Bases6.6 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1502432.41214160895735No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1071323.111772700796067No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA1008521.756952085086187No Hit
CAACCAAAAGAGAAGGAGGCACCTCTCGTTTCAAGCCTAGTGACCTCGAT11812.5478394062951697No Hit
CAACCAAAAGAGAAGGAGGCACTCTGGGGCTAGCTCACCGTGTCCTGGGC3020.6515220158350054No Hit
CAACCAAAAGAGAAGGAGGCACTAGGCGGAAAGAAGACGTCGTCGTGGCG2760.5954307164584816No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA1890.4077405993139603No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1860.4012685263089768No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1690.3645934459474036No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1530.3300757232541583No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1310.282613854550946No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1250.269669708540979No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG1250.269669708540979No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1050.22652255517442238No Hit
GCATCAATCATCACAGGGTTGGGAGAGGGCTGTGTCTTCCCTCCATGACC1010.217893124501111No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC910.19631954781783273No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA900.1941621901495049No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG900.1941621901495049No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC890.19200483248117706No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG820.17690332880288223No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT720.15532975211960393No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT720.15532975211960393No Hit
CAACCAAAAGAGAAGGAGGCACCGCCTGCCCAGTGACACATGTTTAACGG710.1531723944512761No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA700.15101503678294825No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG650.1402282484413091No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT630.13591353310465343No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT610.13159881776799776No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG610.13159881776799776No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG610.13159881776799776No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA610.13159881776799776No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT590.12728410243134208No Hit
CAACCAAAAGAGAAGGAGGCACGCCTCAAGGAATAGGTTCTCCTAGTGTC570.12296938709468644No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA570.12296938709468644No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG570.12296938709468644No Hit
CAACCAAAAGAGAAGGAGGCACTGATGAAGTCGGTGAAGCTGCTGCAGGC520.11218259875304727No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.11002524108471942No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT500.10786788341639161No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGAGAGATGTCCCTCTT490.10571052574806378No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG480.10355316807973594No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG470.1013958104114081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)