FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59000
Total Bases8.5 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC1745329.58135593220339No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1281721.723728813559323No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA1044817.708474576271186No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA743412.6No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA5931.005084745762712No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2830.4796610169491526No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG2580.43728813559322033No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2080.3525423728813559No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1990.33728813559322035No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1730.29322033898305083No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG1680.2847457627118644No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1650.2796610169491526No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1490.2525423728813559No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1450.24576271186440676No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1440.24406779661016947No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1420.2406779661016949No Hit
GCAAAAGTTCTCAGGGATGTCCGTCTCTACACAGTACTGAAATGGAAAAA1350.2288135593220339No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT1330.22542372881355932No Hit
GCAAAAGTTCTCAGGGATGTCGTGCCATATCTGAATATTCTGAGTTTCTG1150.19491525423728814No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1070.18135593220338983No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA1040.17627118644067796No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1040.17627118644067796No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA990.16779661016949152No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG970.16440677966101694No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA890.15084745762711865No Hit
GCAAAAGTTCTCAGGGATGTCCTGGGGGCTGTATAGCCCACTCTGGCCTG850.1440677966101695No Hit
GCAAAAGTTCTCGGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC830.14067796610169492No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA820.13898305084745763No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC810.13728813559322034No Hit
GCAAAAGTTCTCAGGGATGTCCTGTGAGGGGTGGTGGGTGGCGGGACGTC780.13220338983050847No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG740.12542372881355932No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA740.12542372881355932No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC730.12372881355932203No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA720.12203389830508474No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC690.11694915254237287No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG680.1152542372881356No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG620.10508474576271186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)