FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707
Total Bases245.9 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT71041.59343878148799No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT46327.123608670181603No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC311.8160515524311658No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA271.5817223198594026No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA241.4059753954305798No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT231.3473930872876392No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC150.8787346221441126No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.6444053895723492No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.6444053895723492No Hit
GCATCAATCATCACAGGGTTGCTCCATCAAAGAGAGACAGCACGAATCCT100.5858230814294083No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.46865846514352666No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA80.46865846514352666No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC80.46865846514352666No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA70.4100761570005858Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA60.35149384885764495No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC60.35149384885764495No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.35149384885764495No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT50.29291154071470415No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.29291154071470415No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.29291154071470415No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC50.29291154071470415No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.29291154071470415No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG40.23432923257176333No Hit
TCCTATCAGTGGCCGCAAAGGGGGGAGAGGCCCTGGGCTTCACTTACTCT40.23432923257176333No Hit
CAACCAAAAGAGAAGGAGGCACAGTCCATCAAAGAGAGACAGCACGAATC40.23432923257176333No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG40.23432923257176333No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT30.17574692442882248No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG30.17574692442882248No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT30.17574692442882248No Hit
CAATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG30.17574692442882248No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.17574692442882248No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG30.17574692442882248No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA30.17574692442882248No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGAGAGATGTCCCTCTT30.17574692442882248No Hit
GCATCAATCATCACAGGGTTGGTATCAACGCAGAGTACATGGGGTGGTAT30.17574692442882248No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT30.17574692442882248No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT30.17574692442882248No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT30.17574692442882248No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT30.17574692442882248No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT30.17574692442882248No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGG30.17574692442882248No Hit
CAACCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG20.11716461628588166No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT20.11716461628588166No Hit
CAACCAAAAGAGAAGGAGGCACATTCCATCAAAGAGAGACAGCACGAATC20.11716461628588166No Hit
CAACCAAAAGAGAAGGAGGCACACAGAGGGTGTGATATCGAAACGACGCA20.11716461628588166No Hit
TCCTATCAGTGGCCGCATAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.11716461628588166No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTATT20.11716461628588166No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.11716461628588166No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.11716461628588166No Hit
GCATAAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT20.11716461628588166No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTT20.11716461628588166Clontech Universal Primer Mix Long (100% over 23bp)
GCATCAATCATCACAGGGTTGGTATCAACGCAGAGTTGCTTACATCCCTG20.11716461628588166No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.11716461628588166No Hit
TCCTATCAGTGGACGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.11716461628588166No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.11716461628588166No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.11716461628588166No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC20.11716461628588166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.11716461628588166No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT20.11716461628588166No Hit
TCCTATCAGTGGCCGAAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.11716461628588166No Hit
TCCTATCAGTGGCCGCAAAGAGGGAGAGGCCCTGGGCTTCACTTACTCTT20.11716461628588166No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTACACTTACTCT20.11716461628588166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)