FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161
Total Bases458.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT204564.69471686175261No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA37911.989876621322367No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT631.9930401771591266No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA621.9614046187915217No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC401.2654223347042075No Hit
CAACCAAAAGAGAAGGAGGCACTCCAGTGCTGAAGTTTGTTATGCTGGGT220.6959822840873141No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT200.6327111673521038No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC200.6327111673521038No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC190.6010756089844985No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT120.37962670041126223No Hit
CAACCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG110.34799114204365705No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.34799114204365705No Hit
CAACCAAAAGAGAAGGAGGAAAGGAGGAAGAGACTTTTATGTCGGCGGAC90.2847200253084467No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC90.2847200253084467No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA70.22144890857323632No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC70.22144890857323632No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA70.22144890857323632No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA70.22144890857323632No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.18981335020563112No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA60.18981335020563112No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT60.18981335020563112No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT60.18981335020563112No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT60.18981335020563112No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.15817779183802594No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT50.15817779183802594No Hit
CAATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG50.15817779183802594No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT50.15817779183802594No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.15817779183802594No Hit
CAACCAAAAGAGAAGGAGGCACAGCGGCAGCTGGAGCAGGAGCGCCGGGA40.12654223347042076No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.12654223347042076No Hit
CAACCAAAAGAGAAGGAGGCCAGAAATTAATTAATAAGCCCATGGTGGGT40.12654223347042076No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACGTACTCT40.12654223347042076No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT40.12654223347042076No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT40.12654223347042076No Hit
CAACCAAAAGAGAAGGAGGCGTAGGCTGAGGAGGAAGAGGGAGGAGGGGT40.12654223347042076No Hit
GGGCAAAGGGTAAAGGGCAAATGAACAAAATGAAAGGAGGCAAGGGCTGC40.12654223347042076No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACATACTCT40.12654223347042076No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.12654223347042076No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT40.12654223347042076No Hit
CAACCAAAAGAGAAGGAGGCACTGAACCCCAGTGGGGAGACCAGAAGTGC40.12654223347042076No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)