FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11167
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA368633.00796991134593No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT250122.396346377720068No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT221419.826273842571865No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA7516.725172382913942No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT670.5999820900868631No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG490.4387928718545715No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG440.39401808901226826No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG310.2776036536222799No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA290.25969374048535865No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG260.23282887077997672No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG240.21491895764305544No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC230.20596400107459478No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.19700904450613413No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG180.16118921823229157No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.15223426166383092No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.15223426166383092No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.15223426166383092No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.15223426166383092No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC160.1432793050953703No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG160.1432793050953703No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC160.1432793050953703No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA160.1432793050953703No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA150.13432434852690964No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.13432434852690964No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.125369391958449No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG140.125369391958449No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT130.11641443538998836No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG120.10745947882152772No Hit

[OK]Adapter Content

Adapter graph