FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72758
Total Bases10.4 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA3483947.88339426592265No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT2160529.694329145935843No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT25253.4704087523021525No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA18482.539926880892823No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG5310.7298166524643338No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG4900.6734654608427939No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG2930.4027048571978339No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT2500.3436048269606091No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG2340.3216141180351302No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA1870.2570164105665356No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1670.22952802440968692No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1550.2130349927155777No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1520.20891173479205039No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1360.18692102586657136No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC1260.173176832788147No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1160.15943263970972266No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1050.14431402732345583No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG880.12094889909013441No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGGCA870.11957447978229198No Hit
CAACCAAAAGAGAAGGAGGCACCACGGGTTTCTGGATCCAGCCCCTGACA860.11820006047444954No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACG850.11682564116660711No Hit
CAACCAAAAGAGAAGGAGGCACTGGCTACAATTAGGCTTCTCGACGTCCT830.11407680255092224No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT820.1127023832430798No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCATGACA820.1127023832430798No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCCGACA790.1085791253195525No Hit
CAACCAAAAGAGAAGGAGGCACCACAAGTTTCTGGATCCAGCCCCTGACA760.10445586739602519No Hit
CAACCAAAAGAGAAGGGGGCACCACAGGTTTCTGGATCCAGCCCCTGACA740.1017070287803403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)