FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32438
Total Bases4.6 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1106934.12355878907454No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1088633.55940563536593No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA452113.937357420309512No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT1410.4346753807263086No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1400.43159257660768235No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG1300.4007645354214193No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC980.3021148036253776No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA960.29594919538812503No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT950.29286639126949876No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA950.29286639126949876No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG820.2527899377273568No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT810.2497071336087305No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA780.2404587212528516No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG710.21887909242246745No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG660.20346507182933596No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG640.19729946359208334No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA600.18496824711757814No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG590.18188544299895185No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT530.16338861828719403No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG510.15722301004994144No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG480.14797459769406252No Hit
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA470.14489179357543622No Hit
CAACCAAAAGAGAAGGAGGCACTATTAATAATCTTGTCACAGCAAAAGAT470.14489179357543622No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG450.13872618533818362No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG440.13564338121955732No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG440.13564338121955732No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.1294777729823047No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG420.1294777729823047No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT390.1202293606264258No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC390.1202293606264258No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT380.11714655650779951No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA350.10789814415192059No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA340.10481534003329428No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA330.10173253591466798No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT330.10173253591466798No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.10173253591466798No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT330.10173253591466798No Hit

[OK]Adapter Content

Adapter graph