FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392
Total Bases491.7 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA228567.36438679245283No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT852.5058962264150946No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA812.3879716981132075No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA401.179245283018868No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA351.0318396226415094No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT330.972877358490566No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA330.972877358490566No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC260.7665094339622641No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA240.7075471698113208No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC240.7075471698113208No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC190.5601415094339622No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA190.5601415094339622No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.5306603773584906No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA160.4716981132075472No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA160.4716981132075472No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA150.44221698113207547No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA150.44221698113207547No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA140.41273584905660377No Hit
CAACCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG120.3537735849056604No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC110.3242924528301887No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA100.294811320754717No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT100.294811320754717No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA90.2653301886792453No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGA90.2653301886792453No Hit
CAACCAAAAGAGAAGGAGGCTGGAGAGCCAGGACATCCCTGAGAACTTTT80.2358490566037736No Hit
GGGCAAAGGGTAAAGGGCAAATGAACAAAATGAAAGGAGGCAAGGGCTGC80.2358490566037736No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGGAATCCTGTAGAAC70.20636792452830188No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT70.20636792452830188No Hit
GGGCAAAGGGTGAAGAGAGAGAGGGCAAATGAACAAAATGAAAGGAGGCA70.20636792452830188No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGGGGCA70.20636792452830188No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA60.1768867924528302No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAGATGAACAAAATGAAAGGAGGCA60.1768867924528302No Hit
GGGCAAAGGGTGAAGAGAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.1768867924528302No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCGAATCCTGTAG60.1768867924528302No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA60.1768867924528302No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA50.1474056603773585No Hit
CATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAGA50.1474056603773585No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGTAAATGAACAAAATGAAAGGAGGCA50.1474056603773585No Hit
GGGCAAAGGGTGAAGAGAATGAGGGCAAATGAACAAAATGAAAGGAGGCA50.1474056603773585No Hit
GGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.1474056603773585No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGTCA40.1179245283018868No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCGAATCCTGTAG40.1179245283018868No Hit
CAACCAAAAGAGAAGGAGGCAGGACATCCCTGAGAACTTTTGCGAATCCT40.1179245283018868No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA40.1179245283018868No Hit
CAATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG40.1179245283018868No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA40.1179245283018868No Hit
CAACCAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA40.1179245283018868No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGTA40.1179245283018868No Hit
GGGCAAGGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA40.1179245283018868No Hit
CAACCAAAAGAGAAGGAGGCAGGTAGGGTCTCTTGCTTTCATGGAGATTC40.1179245283018868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)