FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32974
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2072462.84951780190453No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA660520.03093346272821No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2420.7339115666889064No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2260.6853884878995573No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCTGGGCTCCTGGTGCTGA1680.5094923272881665No Hit
GCAAAAGTTCTCAGGGATGTGTATGCAGAGGGAAGACCACGTGAGGACAC1640.4973615575908291No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1160.3517923212227816No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1150.34875962879844724No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1020.30933462728210104No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG970.29417116516042946No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA950.28810578031176076No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA950.28810578031176076No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA930.2820403954630921No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG920.2790077030387578No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG710.21532116212773703No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG670.20319039243039969No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA670.20319039243039969No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG620.18802693030872808No Hit
GCAAAAGTTCTCAGGGATGTCGGGATGAAACATCTCACAGGTAAATCTCA620.18802693030872808No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA600.18196154546005944No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG550.16679808333838783No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA540.1637653909140535No Hit
GCAAAAGTTCTCAGGGATGTCCCCACGGCCTGGTCTCTGAATGCGTTGAG520.15770000606538487No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA480.14556923636804756No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA460.1395038515193789No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA460.1395038515193789No Hit
GCAAAAGTTCTCAGGGATGTCCGCCGAGTGGAAGCTGGAGGAATGCGAGT420.12737308182204163No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA400.12130769697337296No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA390.11827500454903864No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA380.1152423121247043No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG370.11220961970037No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA370.11220961970037No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA370.11220961970037No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAACAGCA350.10614423485170134No Hit
GCAAAAGTTCTCAGGGATGTCGCCCAGGCTGAAGTGCAGTGGCATGATCT350.10614423485170134No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA330.10007885000303268No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG330.10007885000303268No Hit
GCAAAAGTTCTCAGGGATGTCCGCGCGTCCACCTCAGAGACGTCATCGCT330.10007885000303268No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA330.10007885000303268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)