FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7045
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG279639.687721788502486No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA256436.39460610361959No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA3254.613200851667849No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT911.2916962384669979No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT821.163946061036196No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA590.837473385379702No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT440.624556422995032No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT300.42583392476933996No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.397444996451384No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT280.397444996451384No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT280.397444996451384No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT270.38325053229240597No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.340667139815472No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.32647267565649396No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT220.312278211497516No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT220.312278211497516No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT140.198722498225692No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT130.18452803406671398No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT130.18452803406671398No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.18452803406671398No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG120.170333569907736No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.170333569907736No Hit
GCAAAATTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT110.156139105748758No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG110.156139105748758No Hit
GCAAAAGTTCTCGGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG100.14194464158977999No Hit
GCAAAAGTTCTCAGGGATGCCCTATTGCTAAGTGGGAGAACTTGCGCCTG90.127750177430802No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA90.127750177430802No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTCGCGCCTG80.113555713271824No Hit
GCAAAAGTTCTCAGGGATGTCCTGCTCTCTGTTCTTCTCCCTGTCCAGGT80.113555713271824No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT80.113555713271824No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.113555713271824No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCCG80.113555713271824No Hit

[OK]Adapter Content

Adapter graph