FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51968
Total Bases7.5 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC1813534.89647475369458No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1685532.43342056650246No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGCAATGAATTTGGTCT635312.22483066502463No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG35986.923491379310345No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA4390.8447506157635467No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2250.43295874384236455No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2030.390625No Hit
GCAAAAGTTCTCAGGGATGTCCTCGGCCATCAGCCTCTTGAGCGCGGTCC1760.33866995073891626No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1650.317503078817734No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGCAATGAATTTGGTCTT1370.26362376847290636No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1240.23860837438423646No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1150.22129002463054187No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1040.20012315270935963No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG900.1731834975369458No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA860.16548645320197045No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT820.15778940886699508No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG800.1539408866995074No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA770.14816810344827586No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG710.1366225369458128No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA700.13469827586206898No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGCAATGAATTTGGTCTT660.12700123152709358No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG660.12700123152709358No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA590.11353140394088669No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA550.10583435960591134No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA530.10198583743842365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)