FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Total Bases369.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA125548.908807482462976No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT72528.254091971940763No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA1013.9360872954014035No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT943.6632891660171474No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG210.8183943881527669No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT170.6625097427903351No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG150.5845674201091192No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG100.3897116134060795No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA100.3897116134060795No Hit
GCATCAATCATCACAGGTTTCTGGATCCAGCCCCTGACAGGCGCAAGTTC60.23382696804364772No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC60.23382696804364772No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA50.19485580670303976No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT50.19485580670303976No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC50.19485580670303976No Hit
CAACCAAAAAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG50.19485580670303976No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG50.19485580670303976No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.1558846453624318No Hit
CAACCAAAAGGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG40.1558846453624318No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATACAGCCCCTGACA40.1558846453624318No Hit
CAACCAAAAGAGAAGGAGGCATCACAGGTTTCTGGATCCAGCCCCTGACA40.1558846453624318No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCAAGCCCCTGACA40.1558846453624318No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA30.11691348402182386No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT30.11691348402182386No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA30.11691348402182386No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT30.11691348402182386No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCACCTGACA30.11691348402182386No Hit
CAACCAAAAGAGAAGGAGGCACCGCAGGTTTCTGGATCCAGCCCCTGACA30.11691348402182386No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC30.11691348402182386No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG30.11691348402182386No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG30.11691348402182386No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCCGCCCCTGACA30.11691348402182386No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGTCCCTGACA30.11691348402182386No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGCCA30.11691348402182386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)