FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26370
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1435654.44065225635192No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA675725.623814941221084No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCGAATCCTGTAG2120.8039438756162306No Hit
CAACCAAAAGAGAAGGAGGCACTCCAGTGCTGAAGTTTGTTATGCTGGGT1570.5953735305271142No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1280.4854000758437618No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1130.42851725445582106No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA1090.4133485020857034No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT1040.3943875616230565No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCGAATCCTGTAG1030.39059537353052715No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC970.3678422449753508No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT900.3412969283276451No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT880.33371255214258627No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA830.31475161167993937No Hit
GCATCAATCATCACAGGGTTGGTCGGGGGGCGCTCGATGTCTTGCAAACT820.3109594235874099No Hit
CAACCAAAAGAGAAGGAGGGACATCCCTGAGAACTTTTGCGAATCCTGTA690.2616609783845279No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC670.2540766021994691No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT640.2427000379218809No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA550.20857034508911643No Hit
CAACCAAAAGAGAAGGAGGCACTTGCCAGGCCCCAGTGTCACTGGGATCC540.20477815699658702No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT540.20477815699658702No Hit
CAACCAAAAGAGAAGGAGGCACGTGGGCCCTGGAGGCTGTGTCCATTCAC540.20477815699658702No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT530.20098596890405765No Hit
CAACCAAAAGAGAAGGAGGCACTCTGAGAGCACCAAGCGCCCGGGGAGGC520.19719378081152825No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT480.1820250284414107No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT460.1744406522563519No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA400.15168752370117558No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT400.15168752370117558No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC400.15168752370117558No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA370.1403109594235874No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT360.13651877133105803No Hit
CAACCAAAAGAGAAGGAGGCACATCCCTGAGAACTTTTGCGAATCCTGTA330.12514220705346987No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC330.12514220705346987No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC320.12135001896094046No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT310.11755783086841107No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA300.11376564277588168No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT300.11376564277588168No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT280.1061812665908229No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA270.10238907849829351No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCATTTACTCT270.10238907849829351No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.10238907849829351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)