FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20720
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT741735.79633204633205No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA347716.78088803088803No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA325015.685328185328185No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT213310.294401544401545No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT1950.9411196911196912No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1480.7142857142857143No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC980.472972972972973No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC960.4633204633204633No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA730.35231660231660233No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA640.3088803088803089No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA620.29922779922779924Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC610.2944015444015444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG590.28474903474903474No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT580.2799227799227799No Hit
CAACCAAAAGAGAAGGAGGCACTGTGGCCGAGAAGCTGGACTGGGCCCGC530.2557915057915058No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA530.2557915057915058No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.24613899613899615No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA470.22683397683397685No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA430.20752895752895753No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC420.20270270270270271No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT410.19787644787644787No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG370.17857142857142858No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.17374517374517373No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG350.16891891891891891No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT340.1640926640926641No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA320.15444015444015444No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC310.14961389961389962No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA310.14961389961389962No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.13996138996138996No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT280.13513513513513514No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.11100386100386099No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.10617760617760619No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG210.10135135135135136No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT210.10135135135135136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)