FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28296
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1798063.54255018377156No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA469116.57831495617755No Hit
GCAAAAGTTCTCAGGGATGTCCAACCCTGTGATGATTGATGCCTGCATAG4541.6044670624823298No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2480.8764489680520215No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2220.7845631891433419No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCACTGCTTCCCA1320.46649703138252757Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG1170.4134860050890585No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG1140.40288379983036465No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCACCCCATGTAC1120.3958156629912355No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1090.38521345773254173No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA950.3357364998586373No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA940.3322024314390727No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG930.328668363019508No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG890.31453208934124965No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA850.30039581566299123No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG650.2297144472716992No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCATCCCATGTAC610.21557817359344078No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA580.20497596833474696No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG550.19437376307605314No Hit
GTGCTGTCTCTCTTTGATGGACTTAACGGGTAATCAAGTGGGAAACTCAG520.18377155781735935No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG510.18023748939779474No Hit
GCAAAAGTTCTCAGGGATGTGCAACCCTGTGATGATTGATGCCTGCATAG500.17670342097823014No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA460.16256714729997174No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG450.1590330788804071No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.15196494204127792No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA420.1484308736217133No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA420.1484308736217133No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA380.1342945999434549No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA360.1272264631043257No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCCCATGTACTCT360.1272264631043257No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAACAGCA330.11662425784563189No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA330.11662425784563189No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA320.11309018942606729No Hit
GTGCTGTCTCTCTTTGATGGAATCAAATCTCCCATCTCTTTATTCACAGG310.1095561210065027No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA310.1095561210065027No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG310.1095561210065027No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA300.10602205258693807No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)