FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42679
Total Bases6.1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1655638.791911713020454No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1128626.443918554792756No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA563313.198528550340919No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA5161.2090255160617633No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG2630.616228121558612No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT2560.5998266126197896No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG2400.5623374493310528No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1410.3303732514819935No Hit
GCATCAATCATCACAGGGTTGCTGGTCGGTCCATCAAAGAGAGACAGCAC1370.32100096065980926No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1160.2717964338433422No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1130.264767215726704No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT1090.2553949249045198No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC1090.2553949249045198No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1030.24133648867124347No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC990.23196419784905928No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA990.23196419784905928No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA990.23196419784905928No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT850.1991611799714145No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCGAATCCTGTAG820.19213196185477635No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA810.1897888891492303No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA790.18510274373813818Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT750.175730452915954No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC720.1687012347993158No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG670.15698587127158556No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA670.15698587127158556No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCGAATCCTGTAG620.14527050774385528No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT600.1405843623327632No Hit
GCATCAATCATCACAGGGTTGGACATCCCTGAGAACTTTTGCGAATCCTG590.13824128962721713No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG460.10778134445511844No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACG450.10543827174957238No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT450.10543827174957238No Hit
GCATCAATCATCACAGGGTTGGTATCAACGCAGAGTTGCTGGTCGGTCCA440.10309519904402635No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA430.10075212633848028No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT430.10075212633848028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)