FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081
Total Bases156.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA21019.42645698427382No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT18917.483811285846436No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT534.902867715078631No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC524.810360777058279No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG454.162812210915819No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG423.885291396854764No Hit
GTGCTGTCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTATT292.682701202590194No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT262.4051803885291396No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT222.0351526364477337No Hit
GCAAAAGTTCTCAGGGATGTGATGATTGATGCCTGCATAGACCAAGTCTC191.757631822386679No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG191.757631822386679No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT171.572617946345976No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG151.3876040703052728No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA151.3876040703052728No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCCTGCATAGACCA141.2950971322849214No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA131.2025901942645698No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA111.0175763182238668No Hit
GTGCTGTCTCTCTTTGATGCTGCATAGACCAAGTCTCTGCTACCGTATTC100.9250693802035153No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT80.7400555041628123No Hit
GTGCTGTCTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTATT80.7400555041628123No Hit
GTGCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT80.7400555041628123No Hit
GTGCTGTCTCTCTTTGATTGCTGCATAGACCAAGTCTCTGCTACCGTATT60.5550416281221091No Hit
GTGCTGTCTCTCTTTGATGGCACTGACGACATGGTTCTACAGGATTCGTG60.5550416281221091No Hit
GTGCTGTCTCTCTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTATTC50.46253469010175763No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC40.37002775208140615No Hit
GCAAAAGTTCTCAGGGATGGCCTCCTTCTCTTTTGGTTGCTGCATAGACC40.37002775208140615No Hit
GTGCTGTCTCTCTTTGATGGAACACTGACGACATGGTTCTACAGGATTCG40.37002775208140615No Hit
GCAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCATA40.37002775208140615No Hit
GTGCTGTCTCTCTTTGATGGAATCCTGACGACATGGTTCTACAGGATTCG40.37002775208140615No Hit
GTGCCTCCTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTATT30.27752081406105455No Hit
GTGCTGTCTCTCTTTGATGGAATACTGACGACATGGTTCTACAGGATTCG30.27752081406105455No Hit
GCAAAAGTTCTCAGGGATGTTAAGCTTGAGTTAGAACCCCAACATTACCA30.27752081406105455No Hit
GTGCCTCCTTCTCTTTTGGTTCTGCATAGACCAAGTCTCTGCTACCGTAT30.27752081406105455No Hit
GCAAAAGTTCTCAGGGATGTCCCAGTTGTATGATTGATGCCTGCATAGAC30.27752081406105455No Hit
GCAAAAGTTCTCAGGGATGTCTCCTTCTCTTTTGGTTGCTGCATAGACCA30.27752081406105455No Hit
GTGCTGTCTCTCTTTGATGGAATCCACTGAGACATGGTTCTACAGGATTC30.27752081406105455No Hit
GTGCTGTCTCTCTTTGATGATTGATGCCTGCATAGACCAAGTCTCTGCTA30.27752081406105455No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC30.27752081406105455No Hit
GTGCCTCCTTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT30.27752081406105455No Hit
GCAAAAGTTCTCAGGGATGTCCTGTCTCTTATACACATCTCCGAGCCCAA20.18501387604070307No Hit
GTGCTGTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTATTC20.18501387604070307No Hit
GTGCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTATT20.18501387604070307No Hit
GTGCTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACC20.18501387604070307No Hit
CTCCAAGTAACGGTGCGGCCACTATAGGACTGCATAGACCAAGTCTCTGC20.18501387604070307No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA20.18501387604070307No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.18501387604070307Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTATT20.18501387604070307No Hit
GCAAAAGTTCTCAGGGATGTCCCTGTGATGATTGATGCCTGCATAGACCA20.18501387604070307No Hit
GTGCCTCCTTCTCTTTTGGTGCTGCATAGACCAAGTCTCTGCTACCGTAT20.18501387604070307No Hit
GTGCTGTCTCTCTTTGATGAATGATGCCTGCATAGACCAAGTCTCTGCTA20.18501387604070307No Hit
TCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTGC20.18501387604070307No Hit
GTGCTGTCTCTCTTTGATGGAATCCACTGACGACATGGTTCTACAGGATT20.18501387604070307No Hit
GTGCTGTTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT20.18501387604070307No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGACTGCATAGACCAAG20.18501387604070307No Hit
GCAAAAGTTCTCAGGGATGTCAAGACTCATATGTATATCTCCTTCTCTTT20.18501387604070307No Hit
GTGCTGTCTCCCTGTGATGATTGATGCCTGCATAGACCAAGTCTCTGCTA20.18501387604070307No Hit
GCAAAAGTTCTCAGGGATGTCCTGTGATGATTGATGCCTGCATAGACCAA20.18501387604070307No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGCTGCATAGACC20.18501387604070307No Hit
GTGCTTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTATT20.18501387604070307No Hit
CTCCAAGTAACGGTGCTTTCTCTTCACCCTTTGCCCCTGCATAGACCAAG20.18501387604070307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)