FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081
Total Bases156.7 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC62

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT24022.201665124884364No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA18817.391304347826086No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA686.290471785383904No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC534.902867715078631No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC454.162812210915819No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC413.7927844588344124No Hit
CAACCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG312.8677150786308974No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA222.0351526364477337No Hit
GCATCAATCATCACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATG191.757631822386679No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC181.6651248843663276No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT171.572617946345976No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC151.3876040703052728No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA151.3876040703052728No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGA141.2950971322849214No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT131.2025901942645698No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT111.0175763182238668No Hit
CATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAGA100.9250693802035153No Hit
CAACAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG80.7400555041628123No Hit
CAACCAAAAGAGAAGGAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA80.7400555041628123No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA60.5550416281221091Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG60.5550416281221091No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGC60.5550416281221091No Hit
CAACAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAGA50.46253469010175763No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCGAATCCTGTAG40.37002775208140615No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGGAT40.37002775208140615No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTAT40.37002775208140615No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTGCGAATCCT40.37002775208140615No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGG40.37002775208140615No Hit
GCATCAATCATACAACTGGGACATCCCTGAGAACTTTTGCGAATCCTGTA30.27752081406105455No Hit
AACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA30.27752081406105455No Hit
CAACCAAAAGAGAAGGAGACATCCCTGAGAACTTTTGCGAATCCTGTAGA30.27752081406105455No Hit
CAACAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG30.27752081406105455No Hit
CAACAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA30.27752081406105455No Hit
GCATCAATCATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTC30.27752081406105455No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGA30.27752081406105455No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGGAATCCTGTAGAAC20.18501387604070307No Hit
CAACCAAAAGAGAGAAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG20.18501387604070307No Hit
CAACCAAAAGAGAAGGAGGCAGCACGAATCCTGTAGAACCATGTCGTCAG20.18501387604070307No Hit
CAACCAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG20.18501387604070307No Hit
CAACCAAAAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAGA20.18501387604070307No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCGAATCCTGTAG20.18501387604070307No Hit
CAACCAAAAGAGAAACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA20.18501387604070307No Hit
CACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA20.18501387604070307No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGAATCCTGTAGAACCATGTCG20.18501387604070307No Hit
TCCTATAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTCG20.18501387604070307No Hit
CAACCAAAAGAGAGGAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG20.18501387604070307No Hit
GCATCAATCATCACAGGGACATCCCTGAGAACTTTTGCGAATCCTGTAGA20.18501387604070307No Hit
CAACCAAAAGAGAAGGAGGCACGCCAGTGTCTGTGGAGCCATCCTTGAAA20.18501387604070307No Hit
TCCTATCAGTGGCCGCACCATTCCATCCCAAACACCGAATCCTGTAGAAC20.18501387604070307No Hit
CAACCAAAAGAGAAGGAGGCTCGAATCCTGTAGAACCATGTCTCAGTGGA20.18501387604070307No Hit
GCATCAATCATCACAGGACATCCCTGAGAACTTTTGCGAATCCTGTAGAA20.18501387604070307No Hit
GCATCAATCATCACAGGGAGACAGCACGAATCCTGTAGAACCATGTCGTC20.18501387604070307No Hit
GCATCATTCATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTC20.18501387604070307No Hit
CAACCAAAAGAGAAGGAGATATACATATGAGTCTTGACATCCCTGAGAAC20.18501387604070307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)