FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4003
Total Bases580.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC97124.25680739445416No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC85721.4089432925306No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC56214.039470397202097No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC47611.89108168873345No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2706.744941294029477No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG501.2490632025980515No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT300.7494379215588309No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA250.6245316012990257No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.4996252810392206No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.47464401698725955No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT190.47464401698725955No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.39970022483137646No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG130.3247564326754934No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.2997751686235324No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.2747939045715713No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.2747939045715713No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2498126405196103No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2498126405196103No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.2498126405196103No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.2498126405196103No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.19985011241568823No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.19985011241568823No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.19985011241568823No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.1748688483637272No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.1748688483637272No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1498875843117662No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA60.1498875843117662No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1498875843117662No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT60.1498875843117662No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.12490632025980515No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.12490632025980515No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.12490632025980515No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12490632025980515No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.12490632025980515No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA50.12490632025980515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)