FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG428346.52906029331885No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT262628.527973927213473No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC6997.59369907658881No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC1531.6621401412275936No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC830.9016838674633352No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG610.6626833242802824No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC590.6409560021727322No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA570.619228680065182No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG390.42368278109722973No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC340.3693644758283542No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG250.27159152634437805No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC200.21727322107550243No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC200.21727322107550243No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG170.1846822379141771No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT160.17381857686040195No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC150.16295491580662683No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG150.16295491580662683No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC150.16295491580662683No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT140.1520912547528517No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT140.1520912547528517No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.1520912547528517No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.1520912547528517No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC130.1412275936990766No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG130.1412275936990766No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT120.13036393264530147No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.13036393264530147No Hit
TGTGTGTGTGTTTCTGTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAA120.13036393264530147No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.13036393264530147No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC110.11950027159152635No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG110.11950027159152635No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG110.11950027159152635No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.10863661053775121No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.10863661053775121No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG100.10863661053775121No Hit
TGTGTGTGTGTTTCTGTGGGTCTCTTTAAGGTTTGGACAGAAGGGTAAAG100.10863661053775121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)