FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC240426.116241173275395No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC166618.09885931558935No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC126613.753394894079305No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC119713.00380228136882No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG6817.398153177620859No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1531.6621401412275936No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT590.6409560021727322No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA550.5975013579576317No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.46713742531233027No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG350.38022813688212925No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT270.2933188484519283No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.26072786529060293No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.24986420423682784No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.2390005431830527No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.2390005431830527No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.22813688212927757No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.21727322107550243No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.21727322107550243No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2064095600217273No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.17381857686040195No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16295491580662683No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16295491580662683No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT150.16295491580662683No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1520912547528517No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1520912547528517No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT140.1520912547528517No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1520912547528517No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1412275936990766No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA130.1412275936990766No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1412275936990766No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA120.13036393264530147No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.13036393264530147No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC120.13036393264530147No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC120.13036393264530147No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.13036393264530147No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11950027159152635No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.10863661053775121No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.10863661053775121No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10863661053775121No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC100.10863661053775121No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10863661053775121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)