FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9407
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC248126.373976825768047No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC186119.783140214733706No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC174518.550015945572447No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG8278.791325608589348No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC3774.007653874774104No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1791.902838311895397No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1501.5945572446050813No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA650.6909748059955353No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT620.6590836611034336No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG490.5208886999043266No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.39332412033592007No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.3614329754438184No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.255129159136813No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.23386839587541194No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.2232380142447114No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA200.21260763261401086No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT190.2019772509833103No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.19134686935260978No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.19134686935260978No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.19134686935260978No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.17008610609120867No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.17008610609120867No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.15945572446050812No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.15945572446050812No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.13819496119910704No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.13819496119910704No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.1275645795684065No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1275645795684065No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.1275645795684065No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.11693419793770597No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11693419793770597No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11693419793770597No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11693419793770597No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC110.11693419793770597No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT100.10630381630700543No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10630381630700543No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10630381630700543No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.10630381630700543No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10630381630700543No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.10630381630700543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)