FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG504852.517686225551394No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT184619.205160216396173No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC8669.00957136912193No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC3293.4228048272992093No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC700.7282563462338744No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC680.7074490220557637No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC620.6450270495214316No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA600.6242197253433208No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG390.4057428214731586No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG340.3537245110278818No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC270.2808988764044944No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC260.27049521431543905No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT260.27049521431543905No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG250.2600915522263837No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC240.24968789013732834No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC240.24968789013732834No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC200.20807324178110698No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC190.19766957969205162No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT180.18726591760299627No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG170.1768622555139409No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC160.16645859342488556No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG160.16645859342488556No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.14565126924677485No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG120.12484394506866417No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC120.12484394506866417No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG120.12484394506866417No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.12484394506866417No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG110.11444028297960883No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG100.10403662089055349No Hit
TGTGTGTGTGTTTCTGTGGGTCTCTTTAAGGTTTGGACAGAAGGGTAAAG100.10403662089055349No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG100.10403662089055349No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)