FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8205
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC176821.5478366849482No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC153918.75685557586837No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC139917.050578915295553No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC122514.929920780012187No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4255.1797684338817795No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT951.157830591102986No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG700.8531383302864107No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT550.6703229737964655No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA520.6337599024984766No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.40219378427787933No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG240.29250457038391225No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.25594149908592323No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.23156611822059717No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.23156611822059717No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.2071907373552712No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.19500304692260814No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.15843997562461914No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.15843997562461914No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.15843997562461914No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14625228519195613No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14625228519195613No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14625228519195613No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.14625228519195613No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC120.14625228519195613No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.14625228519195613No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.14625228519195613No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.1340645947592931No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1340645947592931No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1340645947592931No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1340645947592931No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA110.1340645947592931No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1340645947592931No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.1340645947592931No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1218769043266301No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.1218769043266301No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.10968921389396709No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC90.10968921389396709No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT90.10968921389396709No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.10968921389396709No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10968921389396709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)