FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8645
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC193922.42914979757085No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC167019.317524580682473No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC163318.88953152111047No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG89710.37593984962406No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2953.412377096587623No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1982.290341237709659No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1531.7698091382301908No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA991.1451706188548294No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT770.8906882591093117No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG600.6940427993059572No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.37015615962984383No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.2776171197223829No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.26604973973395024No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA230.26604973973395024No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.21978021978021978No Hit
AAATACCGTTCAGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGAAA190.21978021978021978No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.21978021978021978No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.19664545980335452No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT170.19664545980335452No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1735106998264893No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.1735106998264893No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.16194331983805668No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.16194331983805668No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT140.16194331983805668No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC140.16194331983805668No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.16194331983805668No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT120.13880855986119145No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.13880855986119145No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.13880855986119145No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.13880855986119145No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.12724117987275882No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.11567379988432619No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.11567379988432619No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.11567379988432619No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC100.11567379988432619No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA100.11567379988432619No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.10410641989589359No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGATTGCAAATTGCTGCTGGAACC90.10410641989589359No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC90.10410641989589359No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.10410641989589359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)