FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9812
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC293729.932735426008968No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC187819.139828781084386No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC129513.198124745209947No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC103910.589074602527518No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4504.5862209539339585No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1641.6714227476559314No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1171.1924174480228291No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA1031.049735018344884No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG820.8357113738279657No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.550346514472075No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.3465144720750102No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG330.336322869955157No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2751732572360375No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.254790052996331No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.24459845087647777No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.2242152466367713No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.19364044027721158No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.19364044027721158No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.19364044027721158No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.1732572360375051No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.16306563391765186No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.16306563391765186No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.15287403179779863No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.15287403179779863No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1426824296779454No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA130.13249082755809213No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.13249082755809213No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA130.13249082755809213No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.12229922543823889No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA120.12229922543823889No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.12229922543823889No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.12229922543823889No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11210762331838565No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11210762331838565No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11210762331838565No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11210762331838565No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.10191602119853241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)