FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10353
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC264825.577127402685214No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC218921.1436298657394No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC153314.807302231237324No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG112410.85675649570173No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6306.085192697768763No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2392.308509610740848No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA750.7244277021153289No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG500.4829518014102193No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.42499758524099296No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.35738433304356226No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT320.30908915290254035No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.251134936733314No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA250.24147590070510966No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT230.22215782864870087No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.2028397565922921No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT210.2028397565922921No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2028397565922921No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.1931807205640877No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT200.1931807205640877No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.1931807205640877No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.17386264850767894No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.17386264850767894No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.16420361247947454No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.15454457645127018No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.15454457645127018No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.15454457645127018No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA150.14488554042306578No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC150.14488554042306578No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14488554042306578No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14488554042306578No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1352265043948614No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1352265043948614No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1352265043948614No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.125567468366657No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.125567468366657No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC130.125567468366657No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.125567468366657No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.11590843233845263No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10624939631024824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)