FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8272
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC240129.025628626692455No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC122514.80899419729207No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC121014.627659574468085No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG7569.13926499032882No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC7038.498549323017409No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1411.7045454545454544No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1351.6320116054158607No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA941.1363636363636365No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT470.5681818181818182No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG350.42311411992263054No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.4110251450676982No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.39893617021276595No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT230.27804642166344296No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA200.24177949709864605No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA180.21760154738878143No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.21760154738878143No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.19342359767891684No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.18133462282398452No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.18133462282398452No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.18133462282398452No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.16924564796905223No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1571566731141199No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1571566731141199No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14506769825918764No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.14506769825918764No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14506769825918764No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.13297872340425532No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA110.13297872340425532No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC110.13297872340425532No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12088974854932302No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.10880077369439071No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.10880077369439071No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10880077369439071No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA90.10880077369439071No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10880077369439071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)