FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11149
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC313628.12808323616468No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC217819.535384339402636No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC163914.700870033186833No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC8177.328011480850301No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG7146.404161808233923No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2252.0181182168804375No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1411.264687415911741No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1331.1929321015337697No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA1211.085299129966813No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.4574401291595659No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.43950130056507314No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG460.4125930576733339No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.25114360032289895No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.25114360032289895No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA240.21526594313391337No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.21526594313391337No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.20629652883666694No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.1973271145394206No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.1883577002421742No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.1883577002421742No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.17938828594492778No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1704188716476814No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.161449457350435No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.15248004305318863No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.14351062875594225No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.14351062875594225No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA150.13454121445869585No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.13454121445869585No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.12557180016144948No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA140.12557180016144948No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.12557180016144948No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT140.12557180016144948No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.11660238586420307No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10763297156695668No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10763297156695668No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10763297156695668No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.10763297156695668No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10763297156695668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)