FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8492
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC245628.921337729627883No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC179721.161092793217147No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC171120.148374941121055No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4645.463966085727743No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2613.073480923221856No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG770.9067357512953367No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA600.7065473386716911No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT430.5063589260480452No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.41215261422515315No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.40037682524729157No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG260.30617051342439944No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT250.2943947244465379No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2826189354686764No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.2826189354686764No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.23551577955723035No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.23551577955723035No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2237399905793688No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.2119642016015073No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.2119642016015073No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.2119642016015073No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.2119642016015073No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.18841262364578426No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.18841262364578426No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.18841262364578426No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.17663683466792277No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.15308525671219972No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.15308525671219972No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA130.15308525671219972No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.15308525671219972No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1413094677343382No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.1413094677343382No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.1413094677343382No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.1413094677343382No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1413094677343382No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1295336787564767No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC110.1295336787564767No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT90.10598210080075365No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC90.10598210080075365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)