FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132
Total Bases743.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC169032.93063133281372No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC107520.946999220576775No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC72314.088074824629773No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4999.723304754481683No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG991.9290724863600937No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA440.857365549493375No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.6819953234606391No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT280.5455962587685114No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.46765393608729544No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG220.4286827747466875No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.35074045206547155No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.3117692907248636No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.2727981293842557No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.23382696804364772No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.23382696804364772No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA120.23382696804364772No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.23382696804364772No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.21434138737334374No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.21434138737334374No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.19485580670303976No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.19485580670303976No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.17537022603273578No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA90.17537022603273578No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT90.17537022603273578No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.1558846453624318No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.1558846453624318No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.13639906469212784No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT70.13639906469212784No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT70.13639906469212784No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT60.11691348402182386No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.11691348402182386No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.11691348402182386No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC60.11691348402182386No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATATCAATTAAATAC60.11691348402182386No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.11691348402182386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)