FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8497
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC221826.103330587266093No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC167619.72460868541838No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC122714.440390726138638No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG86010.121219253854303No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6407.5320701424032No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1651.9418618335883253No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT951.1180416617629751No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA770.9062021890078851No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG390.45898552430269507No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.35306578792515003No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT300.35306578792515003No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.30599034953513005No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.28245263034012No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA220.25891491114511No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.22360833235259503No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.22360833235259503No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.21183947275509002No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.21183947275509002No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.200070613157585No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.200070613157585No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.18830175356008003No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.18830175356008003No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.16476403436507003No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT140.16476403436507003No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.16476403436507003No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA140.16476403436507003No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT140.16476403436507003No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.15299517476756502No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.15299517476756502No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA110.129457455572555No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.11768859597505No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.11768859597505No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.10591973637754501No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10591973637754501No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA90.10591973637754501No Hit
AAATACCGTTCAGAGCTGCCCACAGAAACACACACACAACCGAATGTAGA90.10591973637754501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)