FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6891
Total Bases998.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC151922.04324481207372No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC132419.213466840806852No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG107115.542011319111884No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC73210.62255115367871No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC4616.689885357712959No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2403.48280365694384No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG761.1028878246988827No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA721.0448410970831519No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.391815411406182No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT270.391815411406182No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT180.26121027427078797No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.24669859236685532No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC160.23218691046292267No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.21767522855899No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT150.21767522855899No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT150.21767522855899No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.18865186475112464No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.18865186475112464No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.18865186475112464No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.174140182847192No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1596285009432593No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.1596285009432593No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1596285009432593No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1596285009432593No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC110.1596285009432593Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.14511681903932666No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.14511681903932666No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.14511681903932666No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.14511681903932666No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.13060513713539398No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT90.13060513713539398No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC90.13060513713539398No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA90.13060513713539398No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.13060513713539398No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.11609345523146133No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA80.11609345523146133No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.11609345523146133No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT80.11609345523146133No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT80.11609345523146133No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.11609345523146133No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.10158177332752867No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA70.10158177332752867No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.10158177332752867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)