FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948
Total Bases427.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC67422.862957937584802No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC63121.404341926729987No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC50016.960651289009498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC30510.345997286295795No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2077.021709633649932No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG260.8819538670284939No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT250.8480325644504748No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA160.5427408412483039No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT140.47489823609226595No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.40705563093622793No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.30529172320217096No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.27137042062415195No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.27137042062415195No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.23744911804613297No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.23744911804613297No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.23744911804613297No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.20352781546811397No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA60.20352781546811397No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.20352781546811397No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.20352781546811397No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.20352781546811397No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20352781546811397No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.16960651289009498No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC50.16960651289009498No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.16960651289009498No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG50.16960651289009498No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.16960651289009498No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.13568521031207598No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA40.13568521031207598No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13568521031207598No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13568521031207598No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.13568521031207598No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.13568521031207598No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT40.13568521031207598No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA30.10176390773405698No Hit
TGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC30.10176390773405698No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10176390773405698No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC30.10176390773405698No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.10176390773405698No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10176390773405698No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.10176390773405698No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.10176390773405698No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10176390773405698No Hit
AAATACCGTTCAGAGCTGCCCACAGAAACACACACACAACCGAATGTAGA30.10176390773405698No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10176390773405698No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTGAATAC30.10176390773405698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)