FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10553
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC367234.79579266559272No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC155014.687766511892352No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC150314.242395527338198No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG8247.80820619728987No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC5485.192836160333555No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1691.601440348716005No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA840.7959821851606178No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA530.5022268549227707No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG410.3885151141855397No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.3790391357907704No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT370.3506112006064626No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.34113522221169335No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.33165924381692413No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.32218326542215486No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.25585141665877004No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC270.25585141665877004No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.25585141665877004No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA240.22742348147446223No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.22742348147446223No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.217947503079693No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA230.217947503079693No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT220.20847152468492372No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.19899554629015445No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.19899554629015445No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.16109163271107743No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT160.15161565431630816No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.15161565431630816No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT140.13266369752676965No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC140.13266369752676965No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.12318771913200038No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA130.12318771913200038No Hit
GGCACTAGAATGGACCTCTTCCTCATCAATACATTATTTCAATTAAATAC130.12318771913200038No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC130.12318771913200038No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.12318771913200038No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.11371174073723112No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.11371174073723112No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.11371174073723112No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.11371174073723112No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.10423576234246186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)