FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8826
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC251028.43870382959438No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC184720.92680716066168No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC141616.04350781781101No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG5926.707455245864491No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5346.0503059143439835No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1051.1896668932698844No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA550.6231588488556538No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG440.49852707908452304No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA390.4418762746430999No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT360.4078857919782461No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.39655563108996145No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.30591434398368456No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT270.30591434398368456No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.29458418309539997No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.27192386131883073No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.2605937004305461No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.24926353954226152No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.24926353954226152No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2379333786539769No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2379333786539769No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.21527305687740766No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC190.21527305687740766No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.20394289598912305No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.19261273510083843No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.19261273510083843No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.1812825742125538No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.1812825742125538No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT150.1699524133242692No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1586222524359846No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14729209154769998No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA130.14729209154769998No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.14729209154769998No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.13596193065941536No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.13596193065941536No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.12463176977113076No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA110.12463176977113076No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT110.12463176977113076No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.11330160888284614No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.10197144799456152No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10197144799456152No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.10197144799456152No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC90.10197144799456152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)