FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG338144.89443632983667No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT241432.05417607223476No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC4536.015137431947949No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC740.9826052317089363No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG680.9029345372460496No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA670.8896560881689018No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC530.7037578010888329No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG480.6373655557030938No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC420.5576948612402072No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG270.3585181250829903No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC220.29212587969725134No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC210.2788474306201036No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG160.21245518523436463No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.21245518523436463No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG130.17261983800292124No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.17261983800292124No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG120.15934138892577346No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.15934138892577346No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.15934138892577346No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.15934138892577346No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT110.14606293984862567No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC110.14606293984862567No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.1327844907714779No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC90.1195060416943301No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG90.1195060416943301No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC90.1195060416943301No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT90.1195060416943301No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.10622759261718231No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT80.10622759261718231No Hit
ACTTAAAAACAAGGCGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.10622759261718231No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT80.10622759261718231No Hit
TGTGTGTGTGTTTCTGTGGGTCTCTTTAAGGTTTGGACAGAAGGGTAAAG80.10622759261718231No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.10622759261718231No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT80.10622759261718231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)