FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10447
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC358234.287355221594716No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC155114.846367378194698No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC127212.175744232794102No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC9619.198813056379821No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG7457.131233847037427No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1571.5028237771609074No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1401.3400976356848857No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT780.7466258255958649No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA660.6317603139657317No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA610.583899684119843No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.2967359050445104No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.28716377907533264No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.27759165310615486No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG290.27759165310615486No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.26801952713697713No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.2297310232602661No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.22015889729108834No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.21058677132191062No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.20101464535273286No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.19144251938355508No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.17229826744519958No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.17229826744519958No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.15315401550684407No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC150.14358188953766632No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.13400976356848857No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA140.13400976356848857No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.13400976356848857No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT140.13400976356848857No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA130.12443763759931081No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC130.12443763759931081No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11486551163013305No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10529338566095531No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT110.10529338566095531No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10529338566095531No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC110.10529338566095531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)