FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8930
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC313535.1063829787234No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC156317.502799552071668No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC137315.375139977603583No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC7097.9395296752519595No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG3353.7513997760358344No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA740.8286674132138858No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT670.7502799552071668No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG530.593505039193729No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.5823068309070548No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT390.43673012318029114No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.33594624860022393No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.3023516237402016No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.25755879059350506No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.24636058230683092No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.23516237402015677No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.23516237402015677No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2127659574468085No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA180.2015677491601344No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.19036954087346025No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.19036954087346025No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.17917133258678614No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16797312430011196No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.15677491601343785No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG140.15677491601343785No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC140.15677491601343785No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.1455767077267637No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1455767077267637No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.12318029115341546No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.12318029115341546No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.12318029115341546No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA100.11198208286674133No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC100.11198208286674133No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.1007838745800672No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC90.1007838745800672No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)