FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9332
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC223023.896270895842264No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC200421.474496356622375No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC190620.42434633519074No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC7337.854693527646807No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4825.1650235747963995No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA920.9858551221603087No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG780.8358336905272181No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT630.675096442348907No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3857693956279469No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT320.34290612944706383No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.32147449635662234No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.24646378054007717No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG230.24646378054007717No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.21431633090441493No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.21431633090441493No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.19288469781397344No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.19288469781397344No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1821688812687527No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1821688812687527No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA170.1821688812687527No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1821688812687527No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.1821688812687527No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1821688812687527No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.17145306472353192No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA160.17145306472353192No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA140.15002143163309042No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1393056150878697No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.12858979854264896No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.12858979854264896No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.12858979854264896No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.12858979854264896No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC120.12858979854264896No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT120.12858979854264896No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.12858979854264896No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11787398199742821No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.11787398199742821No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.11787398199742821No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.11787398199742821No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC100.10715816545220747No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)