FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10709
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC379535.4374824913624No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC267224.95097581473527No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC148213.838827154729666No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG6215.798860771313849No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1541.4380427677654308No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA710.6629937435801662No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA490.45755906247081896No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG370.34550378186572045No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3081520216640209No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.298814081613596No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.2894761415631712No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.2894761415631712No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.2894761415631712No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.28013820151274627No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.2708002614623214No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.24278644131104676No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.24278644131104676No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.2334485012606219No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.21477262115977216No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.21477262115977216No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.20543468110934726No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.1960967410589224No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.1960967410589224No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.1960967410589224No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT190.17742086095807266No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.17742086095807266No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA190.17742086095807266No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.17742086095807266No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1587449808572229No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.149407040806798No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.149407040806798No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14006910075637313No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14006910075637313No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA140.13073116070594828No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT120.11205528060509852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)