FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9314
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC226124.27528451793No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC180219.34721923985398No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC178019.111015675327465No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG8088.675112733519432No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5876.302340562593944No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1831.964784195834228No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA961.0307064633884475No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG450.4831436547133348No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.3757783981103715No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.36504187245007513No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT310.3328322954691862No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC300.3220957698088898No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.2791496671677045No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.2147305132059266No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.2147305132059266No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.20399398754563022No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.20399398754563022No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.20399398754563022No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.20399398754563022No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA180.1932574618853339No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.17178441056474125No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT160.17178441056474125No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.1610478849044449No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.1610478849044449No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1503113592441486No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA140.1503113592441486No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1503113592441486No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.1503113592441486No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA130.13957483358385225No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.12883830792355594No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.12883830792355594No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11810178226325961No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11810178226325961No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1073652566029633No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT100.1073652566029633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)